Part:BBa_K2915300:Design

NaIII-methylase-CBD 6-His-Tag with TEV site

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 4
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We add a 6Hig-Tag onto the "NlaIII-Methylase" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain.

Source

Nucleotide sequence